Table 1.
Multiplex mix information designed for fetal fraction estimation using ddPCR followed by methylation sensitive restriction digestion
| Name | Sequence | FAM:HEX ratio | Chromosomal Location | |
|---|---|---|---|---|
| DMR 1a | Forward | CGTTAAGGTAATGAACGGCG | 1:1 | 1p34.2 |
| Reverse | CCAGACCCGCAGAAAGTG | |||
| Probe | TCGAAAGTTCAGCGCCCC | |||
| DMR 2a | Forward | CTTCCAGCCAAGCGCTG | 2:1 | 1p34.1 |
| Reverse | GTGATGCAAATCCGCTCC | |||
| Probe | CCTCGCTTTACGGAAAGAACGC | |||
| DMR 3 | Forward | ACACGTCCCCACCTATTTGG | 1:1 | 1p32.3 |
| Reverse | TGTGGGGAGGAGAAGTGACA | |||
| Probe | TCTTCTCCGGGACCTGAGGT | |||
| DMR 4a | Forward | AGCCTCCCCTTTCCTGTCT | 1:1 | 1p21.2 |
| Reverse | GGTGCGTGTTTTCTGTGATT | |||
| Probe | AGGAGCGTGCACAGGTCCT | |||
| DMR 5a | Forward | GAAGGAAAGGAGCTTAGGCG | FAM | 1p32.1 |
| Reverse | CGCAACCATCGAAGTTCAATC | |||
| Probe | CCTGGACGGAGCTGAGACAAT | |||
| DMR 6 | Forward | AGGAGAACGCTGAGGTCG | FAM | 1p32.2 |
| Reverse | GCGGACTACCTTAGTGGCAC | |||
| Probe | CAACTGCAGCTCGCGCT | |||
| DMR 7 | Forward | GCCGCCTTCAGTAGCACAA | FAM | 1p21.2 |
| Reverse | AGCCCGTGGCCTTAAATAGGA | |||
| Probe | CTCAAACCGCGCATCTCTGGC | |||
| REF 1a | Forward | GTTGTGATGTTCTTAAGGCAGA | HEX | 1p31.1 |
| Reverse | AATTGGGATTTCCACAGGAG | |||
| Probe | TCTTCATAAAAAGGAAAGTAATGGCA |
a
Markers selected for the final fetal fraction estimation model (FFMM).