Table 2.

Multiplex mix information designed for fetal fraction estimation using chromosome Y multiplex mix (YMM)

Name		Sequence	Location
CHRY1	Forward	CAGTGTATTTGTGGAAATGCCT	Yq11.21
	Reverse	CTAACTTTTCCAGACAGCAGC
	Probe	ACTGTGTAGTGATAAAGACCTGCT
CHRY2	Forward	ACTTCTAGTTTCCTGCTTTAGC	Yp11.31
	Reverse	CCAACTGGTTTAATTTGATGGG
	Probe	GTGCAAATTTTATGAAGTCTTGGCA
CHRY3	Forward	GACCTGCCCCATCTCCAT	Yq11.221
	Reverse	AGGTTGGATGGAAGATGAAGT
	Probe	GTCACAACGACAGTCATCATTTCT
CHRY4	Forward	CCTCCTTTGAATACTTATTTACGATT	Yq11.221
	Reverse	ACTATGTTTTGCAACCTTTGTT
	Probe	AGTCAAGTTATATGAGTATGTTCAACC
CHRY5	Forward	TGTCATCAACATGGGAAGCA	Yq11.221 (two loci)
	Reverse	TATCTCCCTGAGCAGCAACTA
	Probe	TGG TGA GATCTC TGA GGT CT
CHRY6	Forward	CTCATCACCTGAATTTATGTCTATTT	Yq11.222 (two Loci)
	Reverse	GCTGGGTTTGTCTTTAGGT
	Probe	AAA GAC ACTTGT GGG CCT GT
CHRY7	Forward	CGCTTAACATAGCAGAAGCA	Yp11.31
	Reverse	AGTTTCGAACTCTGGCACCT
	Probe	TGTCGCACTCTCCTTGTTTTT
REF1	Forward	GTTGTGATGTTCTTAAGGCAGA	1p31.1
	Reverse	AATTGGGATTTCCACAGGAG
	Probe	TCTTCATAAAAAGGAAAGTAATGGCA