Table 2.
Multiplex mix information designed for fetal fraction estimation using chromosome Y multiplex mix (YMM)
| Name | Sequence | Location | |
|---|---|---|---|
| CHRY1 | Forward | CAGTGTATTTGTGGAAATGCCT | Yq11.21 |
| Reverse | CTAACTTTTCCAGACAGCAGC | ||
| Probe | ACTGTGTAGTGATAAAGACCTGCT | ||
| CHRY2 | Forward | ACTTCTAGTTTCCTGCTTTAGC | Yp11.31 |
| Reverse | CCAACTGGTTTAATTTGATGGG | ||
| Probe | GTGCAAATTTTATGAAGTCTTGGCA | ||
| CHRY3 | Forward | GACCTGCCCCATCTCCAT | Yq11.221 |
| Reverse | AGGTTGGATGGAAGATGAAGT | ||
| Probe | GTCACAACGACAGTCATCATTTCT | ||
| CHRY4 | Forward | CCTCCTTTGAATACTTATTTACGATT | Yq11.221 |
| Reverse | ACTATGTTTTGCAACCTTTGTT | ||
| Probe | AGTCAAGTTATATGAGTATGTTCAACC | ||
| CHRY5 | Forward | TGTCATCAACATGGGAAGCA | Yq11.221 (two loci) |
| Reverse | TATCTCCCTGAGCAGCAACTA | ||
| Probe | TGG TGA GATCTC TGA GGT CT | ||
| CHRY6 | Forward | CTCATCACCTGAATTTATGTCTATTT | Yq11.222 (two Loci) |
| Reverse | GCTGGGTTTGTCTTTAGGT | ||
| Probe | AAA GAC ACTTGT GGG CCT GT | ||
| CHRY7 | Forward | CGCTTAACATAGCAGAAGCA | Yp11.31 |
| Reverse | AGTTTCGAACTCTGGCACCT | ||
| Probe | TGTCGCACTCTCCTTGTTTTT | ||
| REF1 | Forward | GTTGTGATGTTCTTAAGGCAGA | 1p31.1 |
| Reverse | AATTGGGATTTCCACAGGAG | ||
| Probe | TCTTCATAAAAAGGAAAGTAATGGCA |