Table 2.

Information of the top 20 selected genes after ranking all genes based on coexpression with DCM‐associated genes

Gene	Ranking		Number of variants	Type of mutations	Protein	Protein location/function	Expression		Animal studies	Gene tolerance
	Sarcomere	Z‐disk					Heart	Skeletal muscle		pLi	Z‐score
PDLIM5	25	9	5	2/5 LoF	PDZ and LIM domain protein 5	Z‐disk	High	High	KO mouse: DCM	0.01	−0.47
MYOM1	16	37	8	All missense	Myomesin‐1	Sarcomere	High	High	—	0	−0.35
CKM	28	31	4	2/4 LoF	Creatine kinase, muscle	Cytoplasm	Medium	High	—	0.01	0.95
CMYA5	30	23	14	1/14 LoF	Cardiomyopathy associated protein 5	Anchor protein for PKA	Medium	High	—	0	−2.73
PDE4DIP	32	33	27	1/27 LoF	Phosphodiesterase 4D interacting protein	Anchor protein for cAMP‐dependent pathway	Medium	High	—	—	—
TXLNB	33	16	5	All missense	Beta‐taxilin	Promotes motor nerve regeneration	Medium	High	—	0	−0.91
MYLK3	43	92	5	All missense	Myosin light chain kinase 3	Kinase that phosphorolates MYL2	High	Medium	KO mouse: DCM	0.14	−0.25
LMOD3	48	26	6	All missense	Leiomodin‐3	Sarcomere	Low	Low	Only for LMOD2: DCM	0.01	−2.05
MYOM2	55	75	16	All missense	Myomesin‐2	Sarcomere	High	High	—	0	−5.87
MYH11	72	190	7	All missense	Myosin‐11	Sarcomere	Absent	Absent	KO mouse: no cardiac phenotype	1	3.15
XIRP1	73	89	10	All missense	Xin actin binding repeat containing 1	Sarcomere	Low	Low	KO mouse: myopathy	0	−1.7
DMPK	75	131	6	All missense	Dystrophia myotonica protein kinase	Kinase necessary maintaining muscle structure	Medium	Medium	KO mouse: decreased contractility	0.03	1.47
ADCK3	84	74	4	1/4 LoF	aarF domain containing kinase 3	Mitochondrial respiratory chain	Low	High	—	0	−1.6
SORBS1	87	143	7	All missense	Sorbin and SH3 domain containing 1	Involved in cytoskeletal formation	Low	Low	—	0	0.3
TNXB	92	469	34	1/34 LoF	Tenascin XB	Extracellular matrix	Low	Low	—	0.77	3
ALPK3	92	51	12	All missense	Alpha kinase 3	Cardiomyocyte differentiation	Medium	High	—	0	0.31
OBSCN	105	395	200	4/200 LoF	Obscurin	Z‐disk	Medium	High	—	−1	0
SORBS2	109	795	4	All missense	Sorbin and SH3 domain containing 2	Involved in cytoskeletal formation	Medium	Low	—	0.09	0.38
SPEG	111	355	22	All missense	Striated muscle enriched protein kinase	Myocyte cytoskeletal development	Low	Medium	KO mouse: HF	1	6
SYNM	116	84	14	All missense	Synemin	Intermediate filament within the cytoskelet	Low	Medium	KO mouse: HF	0	−0.15
XIRP2	117	128	20	4/20 LoF	Xin actin binding repeat containing 2	Sarcomere	Low	High	—	0	−5

Abbreviations: DCM, dilated cardiomyopathy; KO, knockout; LoF, loss of function; PKA, protein kinase A.