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. 2019 Dec 3;12(2):e10862. doi: 10.15252/emmm.201910862

Figure EV1. Strategy and validation of endothelial cell‐type‐specific deletion of MT1‐MMP in mice.

Figure EV1

  • A
    Strategy for obtaining endothelial cell‐specific MT1‐MMP (Mmp14)‐null mice by crossing MT1‐MMPf/f mice (Gutierrez‐Fernandez et al, 2015) with Cdh5‐CreERT2/+ mice (Wang et al, 2010). The floxed and targeted Mmp14 alleles are illustrated.
  • B
    PCR of lung DNA from MT1f/f and MT1iΔEC mice detecting the targeted (floxed‐out) Mmp14 allele (810 bp).
  • C
    qPCR analysis of relative Mmp14 and Cdh5 mRNA levels in sorted lung endothelial cells from MT1f/f and MT1iΔEC mice. n = 3 mice per genotype. Data are shown as mean ± SEM.
  • D
    Representative maximum‐intensity projection images of staining for MT1‐MMP (red) and CD31 (green) in colon sections from MT1f/f and MT1iΔEC mice. Scale bar, 10 μm.
Data information: Please see Appendix Table S3 for exact P‐values.Source data are available online for this figure.