Figure 2. MT1‐MMP absence from endothelial cells limits deterioration of vascular perfusion and impedes colitis progression.

• A
  Representative maximum‐intensity projection images of whole‐mount distal colons stained for CD31 (green) and IB4 (red) in MT1^f/f and MT1^iΔEC mice left untreated (basal) or treated with 1% DSS for 3 or 7 days. Scale bar, 40 μm.
• B
  Perfusion decreased during 1% DSS‐induced colitis in MT1^f/f and MT1^iΔEC mice; n = 8–12 mice per genotype and condition. Data are shown as mean ± SEM and were tested by one‐way ANOVA with Benjamini and Hochberg post‐test; **P < 0.01.
• C
  Representative H&E‐stained colon sections from MT1^f/f and MT1^iΔEC mice left untreated (basal) or treated with 1% DSS for 3 or 7 days. Arrows indicate crypt destruction. Scale bar, 50 μm.
• D
  Representative second‐harmonic generation (SHG) microscopy images of mucosal plexus in whole‐mount colons from MT1^f/f or MT1^iΔEC mice left untreated (basal) or treated with 1% DSS for 3 or 7 days. Scale bar, 40 μm.
• E
  Disease activity index (DAI, a composite of weight change, stool consistency, and presence of fecal blood) during 1% DSS‐induced colitis in MT1^f/f and MT1^iΔEC mice. Untreated mice were included as a control. n = 19–24 per genotype and condition. Data are shown as mean ± SEM and were tested by two‐way ANOVA with Benjamini and Hochberg post‐test; ***P < 0.001.

Data information: Please see Appendix Table S3 for exact P‐values.Source data are available online for this figure.