Figure EV4. Impaired VEGF‐induced vasodilation of the colonic mucosa capillaries in the absence of endothelial MT1‐MMP .

• A
  Scheme depicting the method implemented for local administration of VEGF to the mouse distal colon via a rectal cannula and in toto macroscopic image of a colorimetric control (bromophenol blue).
• B
  Representative images of staining for CD31 (green) in the mucosa vascular plexus from wild‐type mice treated with saline or with 20 ng of VEGF in 50 μl via a rectal cannula as explained in (A). Scale bar, 25 μm.
• C
  Representative images of staining for CD31 (green) in the mucosa vascular plexus from MT1^f/f or MT1^iΔEC mice after 10 min of rectal administration of 20 ng of VEGF. Scale bar, 25 μm.
• D
  Quantification of capillary diameter in mice analyzed as in (C); n = 5–8 mice per genotype and condition. Data are shown as mean ± SEM and were tested by unpaired t‐test; *P < 0.05.

Data information: Please see Appendix Table S3 for exact P‐values.Source data are available online for this figure.