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A
Lentivirus (LV) construct used to drive expression of MT1‐MMP (MMP14) and green fluorescent protein (GFP) under the SFFV and IRES promoters, respectively. The point mutations introduced to obtain the catalytic (E240A) and signaling (Y573F) mutants are indicated.
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B
Experimental design for i.v. lentiviral injection in mice and induction of mild colitis with 1% DSS.
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C
Western blot of GFP and MT1‐MMP protein expression in colon lysates from MT1iΔEC mice previously injected with mock LV or LV encoding full‐length MT1‐MMP or the E240A or Y573F mutants; mice were sacrificed 3 days after 1% DSS treatment. Actin is included as a loading control.
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D, E
Representative maximum‐intensity projection images of staining for CD31 (gray) in whole‐mount colons from MT1f/f or MT1iΔEC mice previously injected with mock LV (D) or LV encoding full‐length MT1‐MMP or the E240A or Y573F mutants (E); mice were sacrificed 3 days after 1% DSS treatment. Scale bar, 40 μm. Arrows, arrowheads, and asterisks indicate duplications, loops, and pillars, respectively.
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F
Quantification of IA events in mice treated as in (D and E); n = 5–8 mice per genotype and condition. Data are shown as mean ± SEM and were tested by one‐way ANOVA with Benjamini and Hochberg post‐test; *P < 0.05, **P < 0.01, ***P < 0.001.
‐values.
