Fig. 2.

Higher frequency of CD68⁺CD206⁺ macrophages in PSC liver.

(A) Gating strategy for intrahepatic CD68⁺CD206⁺ macrophages. (B) Representative flow cytometry dot plots showing the populations of macrophage subsets (CD68⁺CD206⁺) from explanted human livers, including, PSC (n = 15), PBC (n = 5), ALD (n = 7) and NL (n = 4); Gated region: CD68⁺CD206⁺ populations. (C,D) The percentages of CD68⁺CD206⁺ macrophages in PSC, other diseased livers (PBC and ALD, n = 12) and NL were analyzed by flow cytometry. (E) The frequency of anti-inflammatory macrophages was negatively associated with liver fibrosis. Data are represented as median ± IQR. *p ≪0.05 with respect to NL. r = -0.6964. One-Way ANOVA with multiple comparisons was used for statistical analysis.

ALD, alcohol-related liver disease; NL, normal liver; PBC, primary biliary cholangitis, PSC, primary sclerosing cholangitis.