Fig. 3.

Frequencies of monocyte subsets present in the circulation and those isolated from human diseased livers.

Monocytes were divided into classical monocytes (C, CD14⁺⁺CD16^-), intermediate monocytes (I, CD14⁺⁺CD16⁺) and non-classical monocytes (N, CD14^dim CD16⁺⁺); (A) Gating strategy for true monocyte subsets. (B) Dot plots showing the CD16⁺ monocyte populations in circulation (top panels) and liver (bottom panels). PSC (left) and PBC (right). (C) Frequencies of circulatory (n = 52) and intrahepatic (n = 21) monocyte subsets, classical vs. CD16⁺ (I+N) monocytes, from patients with PSC, PBC and ALD (intrahepatic monocytes); (D) Comparison of the frequencies of intrahepatic classical and CD16+ monocyte populations between PSC (n = 15) and other diseased livers (PBC, n = 5; and ALD, n = 9). Data are represented as median ± IQR. Mann-Whitney U test was used for statistical analysis. *p ≪0.05; **p ≪0.005. (I+N) denotes total CD16⁺ monocyte population. ALD, alcohol-related liver disease; PBC, primary biliary cholangitis, PSC, primary sclerosing cholangitis.