Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 7;11:764. doi: 10.1038/s41467-020-14629-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — a Boxplots of RPKM values for insulin (INS) and IGF ligands (IGF1, IGF2), and cognate receptors in human blastocyst lineages (EPI green, PE red, TE blue) determined by single-cell RNA-seq¹⁰. Boxes symbolise first and third quartiles, horizontal lines the median, whiskers extend to 1.5 times the interquartile range, dots represent outliers. b Representative phase-contrast images of hESCs in KSR + FGF2 on MEFs, or mTeSR1 or AI medium on laminin-511. n = 2 or 3 biological and 3 technical replicates. Scale bar: 300 μm, top row; 200 μm bottom row. c Flow cytometry of AI-adapted Shef6 hESCs for the indicated proteins (blue); isotype control in grey. n = 3 technical replicates. d Representative immunofluorescence of AI-adapted Shef6 hESCs for the indicated proteins; DAPI (blue) nuclear stain. n = 3 biological replicates. Scale bar: 100 μm. e Upper panels: Representative image of a human day 6 blastocyst prior to ICM (circled) dissection and plating in AI medium on MEFs. The resulting ICM outgrowth (circled) was passaged onto laminin-511 to establish a stable hESC cell line (CH2). Scale bar: 150 μm. n = 3 biological replicates. Lower panels: Representative images of iPSCs derived following reprogramming of BJ fibroblasts with Sendai viruses. iPSC-like colonies (circled) could be detected in AI medium within 12 days of induction, and expanded for picking within 18 days to propagate an established iPSC line (CHiP1). n = 2 technical replicates. Scale bars, left to right: 300 μm, 100 μm, 100 μm. f–h Representative immunofluorescence of AI-cultured hESCs following directed differentiation. n = 3 biological replicates. DAPI in blue. f hESCs differentiated towards hepatocyte-like cells first express endoderm markers CXCR4 (green) and SOX17 (red), upregulate FOXA2 (green) as foregut endoderm, then AFP (red) and CK18 (green) as hepatocytes. Scale bar: 100 μm. g Expression of CTNT (green; scale bar 50 μm), α-Actin (green; scale bar 100 μm) and NKX2.5 (red; scale bar 100 μm) following directed differentiation towards cardiomyocyte-like cells. h Expression of neuronal markers OTX2, PAX6, TUJ1 (green) and NESTIN (red) following directed differentiation towards neuronal progenitor cells. Scale bar: 100 μm.