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. 2020 Feb 7;10:2154. doi: 10.1038/s41598-020-59219-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of MMP-1 on AFL of mouse knee cartilage. Fresh mouse knee joints (n = 5) were incubated in the buffer containing with 50 µg/ml recombinant active human MMP-1 at 37 °C for 24 hours. (A) Tissues treated with MMP-1 were analysed histochemically in comparison to the control tissues without MMP-1 treatment as indicated. Samples were stained with Safranin-O to stain proteoglycans in cartilage tissue (stained red) against counter staining of haematoxilin (purple). The data are representative images from each group. (B) Treated tissues were subjected to measurement of autofluorescence lifetime (AFL). The graph indicates the mean autofluorescence lifetime (mean AFL).*p > 0.05, **p > 0.01.