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. 2020 Feb 7;10:2103. doi: 10.1038/s41598-020-58872-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of viniferifuran (a) and (-)-hopeaphenol (b) on Y. pseudotuberculosis is irreversible. Western blot analysis of the reversibility of viniferifuran and (-)-hopeaphenol treatment of Y. pseudotuberculosis. Lanes 1–4 show the secretion profile of bacteria pretreated with DMSO and lanes 5–8 bacteria pretreated with viniferifuran or (-)-hopeaphenol. After pretreatment the two cultures were divided into four, washed and two of them were diluted in lysogeny broth (LB) supplemented with Ca²⁺ (non-inducing conditions) and the other two were diluted in Ca²⁺ depleted LB (inducing conditions). (-)-Hopeaphenol, viniferifuran (40 µM final concentration) or DMSO were then added and subsequetly the protein secretion profiles were analysed.