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. 2020 Feb 7;10:2103. doi: 10.1038/s41598-020-58872-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inhibition of ExoS expression by (-)-hopeaphenol (1), viniferifuran (8), dehydro-δ-viniferin (10), anigopreissin A (11) and resveratrol-piceatannol hybrid (12). Wild-type P. aeruginosa PAK(pCS500) with GFP under the control of the exoS promoter was treated with different concentration of compounds under T3SS-inducing contitions. As controls the the untreated PAK(pCS500) (wt) and PAKexsA(pCS500) (exsA⁻) were used. The expression of exoS was calculated as percenteage of the untreated wild-type PAK(pCS500). Samples were run in triplicates and the experiment was repeated three times. The graph shows the mean value (n = 3) +/−SD.