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. 2020 Feb 7;10:2168. doi: 10.1038/s41598-020-58901-y

Figure 3.

Figure 3

Deletion of Asic5 does not affect type II UBC density, size or capacitance. (A) Representative Z-projected confocal images of mGluR1α positive type II UBCs immuno-labeled with anti-mGluR1α (red) antibody in lobule X of cerebellar cryosections prepared from control (left) and Asic5 KO (right) mice. Summary graphs of mGluR1α- and GFP-positive type II UBC mean densities (B) and circumferences (C), respectively, in cerebellar sections from wild type (black bars) and Asic5 KO (gray bars) mice. Summary data for type II UBC density from 9 (3 mice, mean age P60) control and 15 (3 mice mean age P60) knockout cryosections, respectively. Summary data for type II UBC circumference from 53 littermate (5 different cryosectons from 2 different animals) and 58 knockout (7 different cryosections from 2 different animals) type II UBCs. (D) Summary graph of GFP-positive type II UBC capacitance in cerebellar sections from wild type (black bars; n = 9, 7 slices, 4 animals, P17 age) and Asic5 KO (gray bars; n = 9, 8 slices, 4 animals, P16.8 age) mice. No significant difference was found between wild type and Asic5 KO for any of these measurements using a two tailed unpaired t-test.