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. 2020 Feb 7;10:2110. doi: 10.1038/s41598-020-58865-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fluorescence microscopy images of the cell nucleus (stained with ethidium bromide and observed by the TRITC filter), the cytoplasm (stained with FITC), the autofluorescent particles (observed by the DAPI filter) and the three of them (merged). Study the NBc internalization extent into the cardiomyocytes upon time from 6 (B), 7 (C) and 8 (D) h, with respect to the corresponding PNcs incubation for 14 h (A), as control of internalization efficiency, and the negligible internalization of the NBc in liver (E) and lung (F) cells after 8 hours’ incubation, as control of cell specificity. The scale is 25 μm.