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. 2020 Feb 7;10:2108. doi: 10.1038/s41598-020-58820-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) A schematic displaying the top view (left) and the side view (right) of the parallel-plate device used to determine the electrical properties of MT solutions in the presence of BRB80 buffer with paclitaxel (BRB80T). (B) MTs imaged at 22 µM tubulin concentration using an epi-fluorescence microscope. Scale bar represents 100 μm. (C) Examples of current-voltage sweeps performed at 100 mV/s for BRB80T and BRB80T containing MTs at a 22 µM tubulin concentration. Error-bars represent standard deviations. Data were collected and averaged from three to five experimental sweeps. In spite of the large amount of noise, which is expected in such complex and irregular MT meshworks, (C) is indicative of a superposition of numerous pinched hysteresis loops providing support for the theoretical predictions in the previous sections of this paper.