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Number of horses assayed with each technique.
| Group | PREG | PHYS | FMR |
|---|---|---|---|
| Method | allantochorion/ endometrium | allantochorion/ endometrium | allantochorion/ endometrium |
| RT-qPCR | 13/13 | 11/7 | 10/8 |
| EIA | 5/5 | 9/6 | 8/5 |
| Western blotting | 12/12 | 6/6 | 5/5 |
| Immunohistochemistry | 6/6 | 6/6 | 6/6 |
Abbreviations: PREG, pregnancy; PHYS, physiological parturition; FMR, fetal membrane retention; RT-qPCR, reverse transcription quantitative polymerase chain reaction; EIA, enzyme immunoassay.
