Table 3.
Hydrodynamic Parameters Obtained from Analysis of the SAXS Data for SaZigA and AbZigAa
| protein | state | RG(Guinier) Å | RG(GNOM) Å |
|---|---|---|---|
| SaZigA | apo | 24 (±1) | 30.7 (±0.4) |
| ZnII | ND | 32.8 (±0.2) | |
| GDPNP | 27 (±1) | 32.1 (±0.4) | |
| GDPNP + ZnII | 25 (±1) | 29.9 (±0.5) | |
| GDP | 25 (±1) | 31.3 (±0.4) | |
| GDP + ZnII | 27 (±1) | 32.1 (±0.1) | |
| AbZigA | apo | 30 (±1) | 28.9 (±0.1) |
a
ND, not determined. The optimized value is shown with the standard deviation provided in parentheses. The theoretical monomer molecular weight is 45.0 kDa for SaZigA and 46.1 kDa for AbZigA. The average molecular weight from GNOM is 75.8 kDa for SaZigA and 69.5 kDa for AbZigA, while the average molecular weights from the linear region of the Guinier plot are 47.0 kDa for SaZigA and 70.3 kDa for AbZigA.44 These findings are consistent with the results from size-exclusion chromatography (Figure S10), which reveals an apparent molecular weight of ≈68 kDa, or consistent with a nonspherical or elongated monomeric particle.