Figure 2.

Comparison of Standard and UMI-based PCR protocols. (a,b) A representative GESTALT blood sample was amplified with UMI-tagged primers using either a standard or UMI-based PCR protocol. (a) Variant allele frequencies for the top 20 most common variants in each sample plus the unaltered GESTALT allele. Identical variants are joined; variants missing in either sample are colored white. (b) Scatter plot of variant allele frequencies from the same sample. Linear regression model plotted in red (slope = 0.97, intercept = 0.001, R² = 0.9998).