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. 2020 Feb 7;10:2099. doi: 10.1038/s41598-020-58964-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PCA measures TIMP-1 interactions with CD63 on the cell surface in TIMP-1’s C-terminus and CD63 LEL-dependent manners. (A) Luciferase activity was measured in cell lysates (top panel) or in live cells (bottom panel) of HEK293FT cells transfected with indicated luciferase fusion protein vectors or without transfection. (B) Luciferase activity was measured in cell lysates (top panel) or in live cells (bottom panel) of HEK293FT cells transfected with indicated luciferase fusion protein vectors. PCA for HNF4 dimerization was used as a positive control for interaction after cell compartment disruption (cell lysates) and as a negative control for extracellular biological interactions (live cells). Values shown are the average of at least triplicate measurements. Error bars represent standard deviation. Significance was assessed as described in Materials and Methods with P < 0.05 being considered as significant.