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. 2020 Feb 7;11:770. doi: 10.1038/s41467-020-14533-4

Fig. 2. TBC1D9 is involved in xenophagy during GAS infection.

Fig. 2

a, b Recruitment of the indicated proteins to invading GAS. a Representative confocal images and b quantification of GAS-positive cells. c, d Conversion of LC3-I to LC3-II. c Representative western blot of LC3 during starvation and GAS infection and d quantification of LC3-II/LC3-I ratio. e, f Localization of ULK1 in GAS-infected cells. e Representative confocal images of ULK1 recruitment to ubiquitin-coated GAS and f quantification of ULK1-positive GAS. g Intracellular bacterial CFU at 6 h p.i in WT and TBC1D9-KO HeLa cells. Scale bars, 10 μm. Data in (b) (n > 200 cells per condition), (d, f) (n > 50 bacteria per condition), and (g) represent the mean ± SEM of three independent experiments. P values calculated by two-tailed Student’s t test.