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. 2020 Feb 7;11:771. doi: 10.1038/s41467-020-14529-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — An image acquired at a short dwell time, (a) before and (b) after denoising, and images acquired at (c) two and (d) four times longer dwell times. Notice how the noise level decreases and visualization of ultrastructure improves as the dwell time increases. By including denoising in the pipeline, we can benefit from both high-quality visualization and short dwell times.