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. 2020 Feb 7;10:2100. doi: 10.1038/s41598-020-58873-z

Figure 2.

Figure 2

Coordination mode of methylcob(III)alamin (MeCbl(III)) bound to core-MetECBDB from Dehalococcoides mccartyi strain CBDB1. Free MeCbl(III) in the “base-on” mode is characterized by broad α/β-absorbance bands and characteristic maxima at ~ 487 and 524 nm (violet line). The UV/Vis spectrum of MeCbl(III) in the presence of core-MetECBDB at 1:1 stoichiometry was slightly changed. The absorbance intensities of β- and γ-bands increased and the maximum of the β-band shifted (Δλ = −5 nm) (red line), indicating the “base-off/His-on” binding mode of MeCbl(III).