Table 1.

Demethylation of methylcob(III)alamin (MeCbl(III)) catalyzed by core-MetE_CBDB from D. mccartyi strain CBDB1 in the presence of D,L-homocysteine at different pH values.

pH	MeCbl(III) consumption [µM min−1]	relative activity [%]
5.0	20.53 ± 4.46	82.0
5.5	19.12 ± 3.23	76.4
6.0	25.05 ± 0.37	100
6.5	23.87 ± 1.41	95.3
7.0	18.96 ± 0.07	75.6
7.5	21.61 ± 0.41	86.3
8.0	18.08 ± 1.02	72.3
8.5	17.70 ± 0.88	70.6
9.0	14.23 ± 0.61	56.9