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. 2020 Jan 31;10:1393. doi: 10.3389/fgene.2019.01393

Figure 2.

Figure 2

Mitochondrial DNA maintenance in mutant RNASEH1 fibroblasts. (A) Southern blot of total DNA digested with PvuII from control (C1 and C2) and patient (P) fibroblasts grown in either glucose- or galactose-containing medium. A radioactive probe against mtDNA was used to detect both linearized mtDNA (empty arrowhead) and 7S DNA (filled arrowhead and bracket), while a probe against 18S rDNA was used as loading control. (B) Relative mitochondrial DNA copy number in control (C1 and C2) and patient (P) fibroblasts grown in either glucose- or galactose-containing medium, calculated as the linearized mtDNA/18S rDNA signal ratio. Data are shown as mean ± SD, n = 4, ***p < 0.001. (C) 7S DNA levels in control (C1 and C2) and patient (P) fibroblasts grown in either glucose- or galactose-containing medium calculated as the 7S DNA/linearized mtDNA/18S rDNA signal ratio. Data are shown as mean ± SD, n = 4, Student’s unpaired two-tail t-test, ***p < 0.001. (D) Western blot analysis of mitochondrial proteins involved in mtDNA maintenance in control (C1 and C2) and patient (P) fibroblasts grown in either glucose- or galactose-containing medium. GAPDH was used as loading control.