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. Author manuscript; available in PMC: 2020 Feb 7.
Published in final edited form as: Clin Cancer Res. 2018 Apr 3;24(13):3186–3196. doi: 10.1158/1078-0432.CCR-18-0263

Figure 2. ZIP4 regulated expression of EMT markers in multiple pancreatic cancer cell lines.

Figure 2.

(A) Western blot. Protein levels of ZIP4 and EMT markers detected in Panc-1 siNC and Panc-1 siZIP4 cells. (B) Western blot. Protein levels of ZIP4 and EMT markers detected in AsPC-shV and AsPC-shZIP4 cells. (C) Western blot. Protein levels of ZIP4 and EMT markers detected in Panc-1 vector and Panc-1 ZIP4 cells. (D) Western blot. Protein levels of ZIP4 and EMT markers detected in MIA-V and MIA-ZIP4 cells. All the immunoblotting was repeated at least three times, and the average values were presented. (E) Western blot. Protein levels of ZO-1 and claudin-1 detected in AsPC-1 or Panc-1 cells with ZEB1 blocked. (F) Western blot. Protein levels of ZO-1 and claudin-1 detected in Panc-V and Panc-ZIP4 cells with ZEB1 blocked. (G) CHIP PCR. CHIP binding assay with anti-ZEB1 in AsPC-1 and Panc-1 cells confirmed the binding of ZEB1 to the ZO-1 and claudin-1 promoter regions.