Figure 3.

Behavioral video surveillance of murine activity via HomeCageScan. (A) Schematic of CleverSys Inc. HomeCageScan chamber and camera set-up made up of four separate chambers, each with its own camera for behavioral recording. One mouse/cage was placed into each chamber, up to 4 animals at a time to be analyzed. (B) Enlarged image for detail of the cameras used to record all behaviors attached to each chamber. (C–E) Specific parameters for each standard size cage were manually defined prior to recording in order to ensure accurate analysis: yellow—area of the proximal lateral cage wall, pink—area of the distal lateral cage wall, dark blue—area of the front and back cage walls, light blue—area and height of the bedding, green—food container, red—drinking spout. All three images were taken from the same animal. (C) Still image of “drinking” behavior recorded and analyzed by the software. (D) Still image of “eating” behavior recorded and analyzed by the software. (E) Still image of “grooming” behavior recorded and analyzed by the software.