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. 2020 Feb 7;11:4. doi: 10.1186/s40104-019-0414-x

Table 2.

Analyzed and calculated concentration of nitrogen, total and free amino acids in control (CON), optimal (OPT) and optimal + leucine (OPTLEU) diets1 (g/kg, as-fed basis)

CON OPT OPTLEU
Analyzed Calculated2 Analyzed Calculated2 Analyzed Calculated2
Total
 Dry matter 887.6 889.5 891.5
 Nitrogen 30.0 30.8 22.0 22.4 22.8 22.9
 Arginine 12.3 12.6 7.5 7.8 8.0 7.8
 Histidine 4.9 5.3 3.9 4.3 4.0 4.3
 Isoleucine 8.5 8.1 6.1 6.0 6.4 6.0
 Leucine 16.5 16.7 11.4 11.9 15.9 16.4
 Lysine 11.1 10.4 10.8 10.1 11.1 10.1
 Methionine 2.7 3.1 2.7 3.3 3.1 3.3
 Methionine + cysteine 5.6 6.3 4.8 5.7 5.2 5.7
 Phenylalanine 9.8 9.6 7.5 7.6 7.7 7.6
 Phenylalanine + tyrosine 16.0 15.9 11.9 12.0 12.3 12.0
 Threonine 7.2 7.3 6.4 6.8 6.6 6.8
 Tryptophan 2.5 2.3 1.8 1.9 1.8 1.9
 Valine 9.4 9.0 8.9 8.9 9.2 8.9
Free amino acids
 Arginine 0.3 0.1 0.1
 Histidine 0.7 0.7 0.7 0.7
 Isoleucine 0.1 0.8 0.8 0.8 0.8
 Leucine 0.1 0.1 4.3 4.5
 Lysine 0.2 3.6 3.7 3.7 3.7
 Methionine3 0.7 1.1 0.7 1.1
 Methionine + cysteine 0.7 1.1 0.7 1.1
 Phenylalanine 1.2 1.3 1.2 1.3
 Phenylalanine + tyrosine 0.1 1.2 1.3 1.2 1.3
 Threonine 0.2 2.0 2.0 2.0 2.0
 Tryptophan4 0.5 0.5
 Valine 2.7 2.9 2.7 2.9

1Analyzed values represents average across 3 blocks (feed mixes)

2Calculated values for the total amino acids are based on the amino acids concentration in feed ingredients according to NRC [13], and calculated values for the free amino acids correspond to the dietary inclusion rate in crystalline form

3Addition of DL-Methionine was omitted in one of the 3 blocks, thus reducing the overall free methionine concentration across all 3 blocks. The average free methionine concentration between blocks 1 and 3 was 0.11 and was zero in block 2. Therefore, across blocks 1, 2 and 3, average free Met was 0.07

4Analysis of free tryptophan was not performed