Fig. 2.

ARG-1 is produced in multiple peritoneal myeloid cells at different time points post-infection. a Representative flow cytometry analysis of ARG-1 expression in SSC^highCD11b⁺F4/80⁺, SSC^lowCD11b⁺F4/80⁺ and CD11b⁺CD11c⁺ cells from one infected mouse at 6 months post-infection. b The percentages of SSC^highCD11b⁺F4/80⁺ cells in total peritoneal leukocytes, SSC^lowCD11b⁺F4/80⁺ and CD11b⁺CD11c⁺ cells in SSC-low leukocytes, together with the percentages of ARG-1 expression in corresponding cells were determined by flow cytometry at 3, 6, 9 and 12 months post-infection. The differences were analyzed by a two-way ANOVA. c Representative flow cytometry analysis of ARG-1 expression in CD11b⁺Gr-1⁺Ly-6C⁺Ly-6G⁻, CD11b⁺Gr-1⁺Ly-6C⁻Ly-6G⁺, CD11b⁺Gr-1⁺ and CD11b⁺Ly-6G⁺ cells from one infected mouse at 12 months post-infection. d The percentages of CD11b⁺Gr-1⁺Ly-6C⁺Ly-6G⁻ⁿ, CD11b⁺Gr-1⁺Ly-6C⁻Ly-6G⁺, CD11b⁺Gr-1⁺ and CD11b⁺Ly-6G⁺ cells in SSC-low leukocytes, and the percentages of ARG-1 expression in the corresponding cells were determined by flow cytometry at 3, 6, 9 and 12 months post-infection. The differences were analyzed by a two-way ANOVA. The percentages of ARG-1 expression (red) were confirmed on the basis of isotypic controls (black) in a and c. The data are the result of one representative experiment out of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001