Figure 6.

Effect of AGE, SAC and SAMC on the IL-6 gene expression induced by TNF-α. The cells were untreated (control) or treated with (A) TNF-α (100 ng/ml) alone for 3, 6 or 24 h or in the presence of (B) AGE (1 mg/ml) or (B) SAC, (C) SAC, (D) SAMC (each at 100 µM) for 3 h and total RNA was extracted. The IL-6 mRNA expression level in total RNA was evaluated by RT-qPCR. Each bar in graphs represents the mean ± SD (n=3-6). ^*P<0.05, ^**P<0.01 (Tukey's multiple comparison test) in comparison to the control. S1PC, S-1-propenylcysteine; hβD3, human β-defensin-3; TNF-α, tumor necrosis factor-α; IL-6, interleukin-6; AGE, aged garlic extract; SAC, S-allylcysteine; SAMC, S-allylmercaptocysteine.