Fig. 2.

DELLAs and JAZs coordinately inhibit the transcriptional function of MYB21. a The schematic diagram shows the constructs used in the transient expression assays of b and c. b and c Transient expression assays show that RGA and RGL2 inhibit transcriptional function of MYB21 b and MYB24 c. The GUS reporter and the internal control luciferase (LUC) were cotransformed with the indicated constructs. Data are means (±SE) of three biological replicates. Asterisks represent Student’s t-test significance compared with the MYB21 (**, P < 0.01). d Schematic diagram of the constructs used in transient expression assays in e. e Transient expression assays show that both RGL2 and JAZ1 proteins could significantly repress transcriptional function of MYB21. Data are means (±SE) of three biological replicates. Asterisks represent Student’s t-test significance between pairs indicated with brackets (*, P < 0.05; **, P < 0.01). f and g Effects of jasmonate and gibberellin on the protein stability of MYB21. MYB21 (myc-MYB21) transiently expressed in N. benthamiana was extracted from tobacco leaves, and then incubated without (Mock) or with methyl jasmonate (JA) f, gibberellin (GA) g, for the indicated time (hours). Then myc-MYB21 was detected by immunoblot using anti-c-myc antibody. The PVDF membrane was stained with Memstain to serve as loading control. The original data can be viewed from Additional file 4: Figure S3e-f. h and i Quantitative analysis of myc-MYB21 protein level in f and g. Data are means (±SE) of three biological replicates. Asterisks represent Student’s t-test significance between pairs indicated with brackets (*, P < 0.05; **, P < 0.01). The abundance of myc-MYB21 with mock treatment for 0 h was set to 1.0