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. Author manuscript; available in PMC: 2020 Apr 30.
Published in final edited form as: Methods Enzymol. 2019 Apr 30;624:265–281. doi: 10.1016/bs.mie.2019.04.004

Fig. 5.

Fig. 5

Quantification of recombination after global irradiation. (a) Representative mCherry fluorescence images of 48 hpf embryos after various irradiation conditions. (b) Quantification of mCherry fluorescence in the 3 brightest embryos using ImageJ software. (c) qPCR results after ΔΔCt analysis with comparison of percent recombination to the wild-type Cre recombinase mRNA injected embryos. Adapted with permission from ChemBioChem, 19, 1244–1249. Copyright 2018 John Wiley and Sons.