Fig. 6.

BRD7 suppresses tumor growth and reduces lung metastasis through YB1 in vivo. a, b and c Tumor volume, image and tumor weight of nude mice with MDA231 cells in xenograft model, n = 5 mice per group. Data represent means ± SDs. **, p < 0.01. d Representative image of macroscopic mouse lung tissue in the metastatic tumor model, n = 11 mice per group. e Representative image of lung metastasis samples by H&E staining is shown in control, BRD7 overexpression and YB1 restoration group. Red arrows indicate metastatic tumors, scale bar, 200 μm. The number of metastatic lung nodules of every mouse per group were counted in microscopy. Data represent means ± SDs. **, p < 0.01; ***, p < 0.001. f Primary tumor samples for IHC analysis of the expression of BRD7, YB1, Ki67 in control, BRD7 overexpression and YB1 restoration group, scale bar, 20 μm. h Primary tumor samples for IHC analysis of the expression of EMT markers E-cadherin and vimentin, scale bar, 20 μm