Fig. 2.

Sensitivity of the ΔclpP mutant to hyperosmotic pressure, low pH, oxidative stress, and SDS. a Overnight cultures of the E. faecalis strains were diluted in TSB containing 5% NaCl or with pH 5.5 and then incubated at 37 °C for 16 h, or in TSB containing 2 mM H₂O₂ incubated at 37 °C for 10 h. Three independent experiments were performed, and the data represent means ± SD. b The E. faecalis strains were spotted onto TSB agar plates containing 0.008% SDS and incubated for 24 h at 37 °C. Three independent experiments were performed, and the representative results are shown