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(a, b, c, d) Necrotic tissues and bacteria present in the apical area of the buccal canal (H&E stain, a=40×, b=400× and B&B stain, a=40×, b=400×). (e, f) Lingual canal: tissues in the main and lateral canal (H&E stain, e=40× and B&B stain, f=400×)
