Table 3.

Effects of the RFT1-Hd3a region on 17 components of amino acid content (AAC) detected in the TF6-15 population.

Trait	Phenotype (mean±SE) a		P	A b	R 2 (%) c
	NILZS97	NILMY46
Asp	0.721±0.006	0.762±0.010	0.0007	0.020	39.8
Thr	0.308± 0.003	0.326±0.004	0.0009	0.009	37.6
Ser	0.427± 0.004	0.452±0.005	0.0005	0.013	38.8
Glu	1.543±0.008	1.626±0.015	0.0003	0.042	42.0
Gly	0.183± 0.001	0.193±0.001	0.0002	0.005	50.0
Ala	0.421±0.004	0.447±0.007	<0.0001	0.013	46.3
Cys	0.273±0.003	0.286±0.006	0.0034	0.006	28.9
Val	0.331±0.005	0.359±0.008	0.0033	0.014	26.3
Met	0.047±0.003	0.051±0.005	0.4121
Ile	0.202±0.004	0.221±0.005	0.0063	0.009	22.6
Leu	0.577±0.004	0.614±0.006	<0.0001	0.019	47.1
Tyr	0.165±0.002	0.177±0.004	0.0295	0.006	16.7
Phe	0.382±0.003	0.413±0.005	<0.0001	0.015	54.9
Lys	0.231±0.003	0.250±0.005	<0.0001	0.010	52.1
His	0.167± 0.002	0.180±0.003	<0.0001	0.007	51.3
Arg	0.499±0.003	0.544±0.007	<0.0001	0.023	61.2
Pro	0.204±0.011	0.224±0.005	0.0534

^aNIL^ZS97 and NIL^MY46 are near isogenic lines carrying homozygous alleles from ZS97 and MY46, respectively.

^bAdditive effect of replacing a ZS97 allele with a MY46 allele.

^cProportion of phenotypic variance explained by the QTL effect. R² = V_G/V_P ×100, in which V_G is the variance between the two genotypic groups, and V_P the phenotypic variance.