Table 4.

Effects of the RFT1 gene on 17 components of amino acid content (AAC) detected in the R1 population.

Trait	Phenotype (mean ± SE) a		P	A b	R2(%) c
	NILZS97	NILMY46
Asp	0.733 ± 0.006	0.819 ± 0.007	<0.0001	0.043	58.7
Thr	0.328 ± 0.002	0.362 ± 0.002	<0.0001	0.017	62.8
Ser	0.432 ± 0.003	0.480 ± 0.004	<0.0001	0.024	63.2
Glu	1.495 ± 0.012	1.660 ± 0.012	<0.0001	0.083	59.8
Gly	0.173 ± 0.001	0.192 ± 0.002	<0.0001	0.010	56.5
Ala	0.440 ± 0.004	0.485 ± 0.003	<0.0001	0.023	53.6
Cys	0.321 ± 0.003	0.334 ± 0.004	0.0009	0.006	10.4
Val	0.377 ± 0.003	0.415 ± 0.004	<0.0001	0.019	46.6
Met	0.066 ± 0.002	0.067 ± 0.002	0.6776
Ile	0.238 ± 0.002	0.261 ± 0.002	<0.0001	0.012	45.4
Leu	0.595 ± 0.005	0.662 ± 0.005	<0.0001	0.033	58.2
Tyr	0.202 ± 0.003	0.217 ± 0.005	0.0026	0.007	9.5
Phe	0.392 ± 0.004	0.445 ± 0.004	<0.0001	0.026	57.1
Lys	0.266 ± 0.003	0.292 ± 0.002	<0.0001	0.013	42.6
His	0.181 ± 0.002	0.202 ± 0.002	<0.0001	0.011	55.5
Arg	0.500 ± 0.005	0.570 ± 0.006	<0.0001	0.035	52.5
Pro	0.185 ± 0.006	0.202 ± 0.006	0.0582

^aNIL^ZS97 and NIL^MY46 are near isogenic lines carrying homozygous alleles from ZS97 and MY46, respectively.

^bAdditive effect of replacing a ZS97 allele with a MY46 allele.

^cProportion of phenotypic variance explained by the QTL effect. R² = V_G/V_P ×100, in which V_G is the variance between the two genotypic groups, and V_P the phenotypic variance.