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. Author manuscript; available in PMC: 2020 Dec 6.
Published in final edited form as: Circ Res. 2019 Oct 21;125(12):1055–1066. doi: 10.1161/CIRCRESAHA.119.315343

Figure 4. A20 suppresses renal DC activation during Ang II-dependent hypertension.

Figure 4.

Assessment of expression of co-stimulatory molecules CD40, CD80 and CD86 on CD11c+MHCIIhi DCs isolated from the kidney after 10 days of Ang II. (A) In gating strategy, T cells, B cells, and NK cells were first excluded via negative staining for CD3, CD19, and NK1.1, respectively, based on “fluorescence minus one” (FMO) threshold. Then DCs were identified by co-expression of CD11c and MHCII and parsed for CD40, CD80, or CD86 positivity. (B) Representative flow plots and absolute number of CD11c+MHCIIhi Cells from WT and DC ACT groups. (C-E) Representative flow plots and absolute number of (C) CD40+, (D) CD80+, and (E) CD86+ DCs from WT and DC ACT mice. N=6 mice/group. Statistical significance was assessed using an unpaired Student’ t-test. Data are mean ± SE.