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. Author manuscript; available in PMC: 2021 Jan 31.
Published in final edited form as: Circ Res. 2019 Dec 9;126(3):298–314. doi: 10.1161/CIRCRESAHA.119.315644

Figure 4. FCCP enhances mitophagy in the aorta of aged mice in tissue culture and reduces levels of Parkin, TLR9, MyD88, and IL-6.

Figure 4.

(A-B) Thoracic aortas from aged (16 months of age) mtKiema mice were incubated with mitotracker green and also 10μM of FCCP or vehicle control. The mitotracker green signal (488nm excitation) and mtKeima red signal (561nm excitation) were assessed by fluorescence microscopy (see methods) and the ratio of 561:488 (mitophagy index) is shown in B. (C) Thoracic aortas from 18-month old WT mice were harvested and divided into 5 equal parts and cultured in DMEM+10% FBS supplemented with the indicated concentrations of FCCP for 2h. Lysates were immunoblotted against Parkin, TLR9, MyD88, IL-6, CoxIV, and β-actin. (D-H) Quantification of immunoblots. Each point is a biological replicate. All results are presented as mean ±SEM. Kruskal-Wallis with Dunn’s post-hoc test for D-H. Mann-Whitney U-test for B.