Table 1. Quantity of viral RNA in different water samples from the two groups (mallards and infection control Pekin ducks), presented as virus equivalents per ml (VE/ml) per log10 (H5-specific RTqPCR).
| 0 dpi | 1 dpi | 2 dpi | 3 dpi | 4 dpi | 5 dpi | 6 dpi | 7 dpi | 8 dpi | 9 dpi | 10 dpi | 11 dpi | 12 dpi | 14 dpi | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| H2O | vRNA/VI | vRNA/VI | vRNA/VI | vRNA/VI | vRNA/VI | vRNA/VI | vRNA/VI | vRNA/VI | vRNA/VI | vRNA/VI | vRNA/VI | vRNA/VI | vRNA/VI | vRNA/VI | |
| Mallards | Pool (300l) | −/n.d. | −/n.d. | 4.09/+ | 4.34/− | 3.86/− | 2.55/− | 2.98/− | 2.45/n.d. | −/n.d. | 1.91/n.d. | −/n.d. | −/n.d. | −/n.d. | −/n.d. |
| Trough (14l) | −/n.d. | −/n.d. | −/n.d. | 2.26/n.d. | 4.18/− | 4.51/+ | 2.66/n.d. | −/n.d. | −/n.d. | −/n.d. | −/n.d. | −/n.d. | −/n.d. | ||
| Paddling (20l) | 4.20/− | 3.65/− | 3.71/− | −/n.d. | −/n.d. | −/n.d. | −/n.d. | −/n.d. | −/n.d. | −/n.d. | |||||
| Infection Control | Pool (300l) | −/n.d. | 1.65/n.d. | 3.73/− | 4.47/− | 4.81/− | 4.26/− | 2.63/n.d. | 2.11/n.d. | −/n.d. | |||||
| Trough (14l) | −/n.d. | 3.88/− | 3.99/− | 3.97/− | 2.68/n.d. | 3.88/− | 1.52/n.d. | −/n.d. | −/n.d. |
Notes: In bold: Infectious virus could be propagated in embryonated chicken eggs.
Dpi, days post infection; viral RNA (vRNA) presented as VE/ml per log10; VI: Virus isolation; −negative; + positive; n.d. not done.