Figure 2. . Morphology of Copper-cysteamine nanoparticle and its dark toxicity for HaCat, squamous cell carcinoma and melanoma cells.

(A) Copper-cysteamine nanoparticles (Cu-Cy NP) were spherical with a diameter of about 80 nm under transmission electron microscope. (B) The survival rates of cells were determined by the CCK-8 method after treated by 0.1, 1, 10, 100 or 200 μg/ml of Cu-Cy NPs for 24 h in vitro. No dark toxicity of Cu-Cy NPs is observed in HaCaT cells. The survival rates of XL50 cells and B16F10 cells were reduced at 200 μg/ml of Cu-Cy NPs.

*p < 0.05 compared with the control group.

Cu-Cy NP: Copper-cysteamine nanoparticles; SCC: Squamous cell carcinoma.