Figure 6. . The effect of superparamagnetic iron oxide nanoparticles on liver-specific functions in 1-week long-term primary rat hepatocyte cultures in the chip with the flow (50 μl/h).

Time-course graph (A & C), and bar graph with statistical analyses (B & D) illustrating liver-specific functions (albumin (A & B) and urea (C & D) synthesis) of primary rat hepatocyte cultures in the chip with the flow (50 μl/h). Positive control acetaminophen (40 mM) and SPION (200 μg/ml) were added at the fifth day; the untreated control cells received medium only. Significant difference between treated and untreated cells is denoted as *p-value < 0.01; between APAP-treated and SPION-treated cells is denoted as **p-value < 0.01.

APAP: Acetaminophen.