Figure 7. . Live/dead assay at the seventh day after the seeding of primary rat hepatocytes that illustrates the effect of superparamagnetic iron oxide nanoparticles on the viability of the hepatocyte cultures in the chip with the flow (50 μl/h).

Positive control acetaminophen (APAP; 40 mM) and SPION (200 μg/ml) were added at the fifth day; the untreated control cells received medium only. Left panel: viability of the untreated control cells; middle panel: viability of the APAP treated cells; right panel: viability of the SPION treated cells. Blue: DNA/nuclei of the cells; red: dead cells; image scale bar: 400 μm.

APAP: Acetaminophen; SPION: Superparamagnetic iron oxide nanoparticle.