Figure 3.

Cytokine production of DC's was affected by hBD2 in a TLR- and CCR2-dependent manner. Human mo-DC's and murine BM-DC's were treated with LPS (10 μg/ml) alone or co-incubated with various concentrations of hBD2 (100 μg/ml or 10 μg/ml). Murine BM-DCs were additionally treated with pertussis toxin or the CCR2 inhibitor RS prior to stimulation with LPS or a cytokine cocktail containing TNF-α (0.2 mg/ml), IL-6 (0.2 mg/ml), and IL-1β (0.2 mg/ml). Release of TNF-α in (A) human Mo-DC's and in (B) murine BM-DC's was quantified by ELISA. Release of TNF-α (C,E,G) and IL-10 (D,F,H) in murine BM-DCs was quantified by LEGENDplex. Results are presented as mean ± SEM, n = 3. Statistical test used is one-way ANOVA with Bonferroni post-test.