Figure 3.

NF-κB activation during morphine-exposure and SHIV infection of RM-PBMCs. (A) NF-κB activation during SHIV infection and morphine-exposure of RM-PBMCs with SHIV AD8EO, SHIV 4NF-κB, and SHIV 3NF-κB was determined by detecting translocation of the phosphorylated NF-κB into the nucleus. GAPDH was utilized as a quality control for the extraction of cytoplasmic and nuclear fractions. Relative band intensity was measured with Image Studio Version 4.0 software to determine alterations in NF-κB expression in the (B) nuclear and (C) cytoplasmic fractions.