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. 2020 Feb 3;15:761–777. doi: 10.2147/IJN.S238445

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© 2020 Renu et al.

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In vitro uptake analysis of CS NPs-F by splenocytes and PBMCs. (A) Splenocytes and (B) PBMCs were treated with either medium, RITC dye tagged CS NPs or RITC dye tagged CS NPs-F for 4 h and observed in the red channel under a fluorescent microscope (20× magnification). (C) PBMCs were treated with either medium, soluble F-protein or CS NPs-F for 4 h, immunostained with flagellin specific antibody and cells were examined in green channel under a fluorescent microscope (20× magnification).

Abbreviations: PBMCs, peripheral blood mononuclear cells; RITC, rhodamine B isothiocyanate; F, flagellin; CS NPs, chitosan nanoparticles; CS NPs-F, surface F-protein coated CS NPs.