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. 2020 Feb 7;15(2):e0228647. doi: 10.1371/journal.pone.0228647

Table 6. Effect of the proportion of dietary wheat and pea starch on SCFA concentration (μmol/g of digesta) and composition (%) in the ileal contents of broilers at 28 d of age.

Diets SEM Regression R2 Equation
01 20 40 60 80 100
SCFA concentration (μmol/g)
Total 67.2 78.8 84.2 96.7 93.4 74.7 3.82 Q = 0.025 0.27 y = -0.008x2 + 1.0x + 64.7
Acetic acid 60.2 60.7 63.5 61.3 63.8 59.1 1.11 NS - -
Propionic acid 4.7 6.3 8.4 9.9 9.4 8.1 0.70 L = 0.050 0.17 y = 0.04x + 5.8
Iso-butyric acid 0.5 0.6 0.9 0.4 1.2 1.0 0.13 NS - -
Butyric acid - 1.7 1.5 1.0 2.2 1.4 0.29 NS - -
Iso-valeric acid 0.5 0.8 1.0 0.6 1.1 0.7 0.12 NS - -
Valeric acid 0.4 0.5 0.6 0.4 1.0 0.9 0.14 NS - -
Caproic acid 1.1 0.8 1.1 1.0 1.1 0.8 0.10 NS - -
Lactic acid - 7.4 7.3 22.1 13.6 2.6 3.45 NS - -
SCFA mixture composition (%)
Acetic acid 90.0 78.6 77.3 67.0 72.6 79.2 3.10 NS - -
Propionic acid 6.6 7.8 10.2 10.9 10.4 10.7 0.87 NS - -
Iso-butyric acid 0.7 0.6 1.0 0.4 1.3 1.4 0.14 NS - -
Butyric acid - 2.0 1.6 0.8 2.3 2.0 0.29 NS - -
Iso-valeric acid 0.7 1.0 1.2 0.7 1.2 0.9 0.14 NS - -
Valeric acid 0.5 0.6 0.6 0.3 1.0 1.1 0.15 NS - -
Caproic acid 1.6 1.1 1.3 1.0 1.2 1.1 0.12 NS - -
Lactic acid - 8.4 6.8 19.0 10.1 3.7 2.87 NS - -

1 Diets denominations indicate the percentage of pea starch in the starch fraction of the diet.

SEM: pooled standard error of the mean; L: linear regression P value; Q: quadratic regression P value; NS: not significant; number of replications = 12 male broilers.