Table 10. Effect of the proportion of dietary wheat and pea starch on relative1 mRNA levels of proglucagon, proglucagon-B and peptide tyrosine-tyrosine in jejunum and ileum samples of broilers at 28 d of age.
| Diets | SEM | Regression | R2 | Equation | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 01 | 20 | 40 | 60 | 80 | 100 | |||||
| Jejunum | ||||||||||
| PG | 0.9 | 1.3 | 0.5 | 1.0 | 0.8 | 0.6 | 0.09 | NS | - | - |
| PGB | 0.9 | 1.3 | 0.4 | 0.9 | 0.6 | 0.3 | 0.13 | NS | - | - |
| PYY | 1.2 | 1.5 | 0.8 | 1.0 | 1.0 | 1.0 | 0.08 | NS | - | - |
| Ileum | ||||||||||
| PG | 1.5 | 1.5 | 1.3 | 1.3 | 1.1 | 1.3 | 0.05 | L = 0.047 | 0.08 | y = -0.001x + 1.2 |
| PGB | 0.9 | 0.9 | 0.7 | 0.9 | 0.7 | 0.8 | 0.04 | NS | - | - |
| PYY | 0.9 | 0.7 | 0.8 | 0.7 | 0.7 | 0.7 | 0.07 | NS | - | - |
1 Obtained values of proglucagon, proglucagon B and PYY were normalized by the average of GAPDH and RPS7
2 Diets denominations indicate the percentage of pea starch in the starch fraction of the diet.
PG: proglucagon; PGB: proglucagon-B; PYY: peptide tyrosine-tyrosine; SEM: pooled standard error of the mean; L: linear regression P value; NS: not significant; number of replications = 12 male broilers.