Fig 5. Probability of release is decreased by hydrophobic mutations.

Probability of release was determined using a paired pulse protocol with interpulse intervals of 10, 20, 50, and 100ms. A, Representative traces of paired EJPs for P[syt^WT], P[syt^A-ME], and P[syt^A-ME,FE] with a 20ms interpulse interval. B, Mean paired pulse ratios ± SEM for P[syt^WT], P[syt^A-ME], and P[syt^A-ME,FE] (Two-way Repeated Measures ANOVA with Tukey post-hoc, p < 0.05 = *, p < 0.01 = **, p < 0.001 = ***, p < 0.0001 = ****). Indicated differences are between mutants and P[syt^WT], though the paired pulse ratio was significantly different (p < 0.05) between P[syt^A-ME], and P[syt^A-ME,FE], for all interpulse intervals except 100ms.